PURPOSE:
To determine whether single or combined therapy could influence the glucose and lipid metabolism parameters as well as PPARγ expression in fat and skeletal muscle tissues of metabolically-impaired hypertensive rats.
METHODS:
Spontaneously hypertensive rats (SHRs) were fed a fructose-rich diet over 16 weeks of either exercise training (Ex, 20m/min, 0%grade, 60min/day, 5 days/wk), ACE inhibitor (TM, temocapril, 10mg/kg/day) administration, or a combination of exercise training and ACE inhibitor administration. (TM+Ex).
RESULTS:
The systolic blood pressure was reduced exclusively in the temocapril-treated groups. Epididymal fat pads (EPI) weighed less in TM+Ex < Ex < TM. Serum leptin levels were positively correlated with EPI weights (P < 0.001). Of the two isoforms, only PPARγ1 was present in skeletal muscles, whereas both γ1 and γ2 were found in EPI. Exercise training tended to upregulate the PPARγ expression in all tissues, which was attenuated by TM. Extensor digitorum longus, a poorly oxidative muscle, had a significantly higher abundance of PPARγ protein than highly oxidative soleus muscle.
CONCLUSION:
These data suggested that PPARγ expression is tissue specific and may be influenced by diet, endurance training, and ACE inhibition, or combintions there of.
Kawamura, T; Yoshida, K; Sugawara, A; Nagasaka, M; Mori, N; Takeuchi, K; Kohzuki, M
