A highly sensitive determination method for sulfite (SO32-) in sediments was developed using HPLC with NAM fluorometry. Sediments were collected in organic matter from the rich coast of Japan in the extraction buffer containing EDTA, which effectively prevented the loss of sulfites by oxidation. Sulfite was labeled with N-(9-acridinyl) maleimide (NAM). The concentration was determined on a high performance liquid chromatography (HPLC) with fluorometer. The concentration range of the determination was 1 - 1,000 pmol on column. The good recovery was obtained by using the buffer solution containing EDTA and deoxygenated redistilled water. The determination required less than 100 mg of sediment.
宮城県奥松島の牡蠣養殖漁場での環境汚染調査の一環で、海洋汚泥中に含まれる亜硫酸を蛍光光度法による超微量定量法(1～1000ピコモル)を開発すると共に漁場の各地点における亜硫酸濃度を測定し評価した。環境中の酸化還元条件によって変動する亜硫酸の実態は解明されておらず環境汚染の評価手法の１つとして開発した分析法が有効であることを報告した。
pp.131-134
Masato Yamaguchi, Tsuneo Kamata and Hiroshi Meguro
